Application Guide: Preparation of Media, Buffer & Microbiological Testing
Last updated: June, 2024
Overview
Cell or microbiological media and buffer preparation involve dissolving precise amounts of various salts, nutrients, and buffering agents in ultrapure water, followed by pH adjustment to suit specific organism requirements. Key hurdles to avoid include inaccuracies in weighing and volumetric measurements, which can lead to inconsistent growth conditions, and contamination from non-sterile equipment or reagents, which can introduce unwanted variables into experiments.
Ensuring endotoxin-free and mycoplasma-free conditions is also critical, as such contaminants can significantly impact cell health and experimental outcomes.
Download the documentation in this guide to optimize buffer and media preparation without contributing contaminants.
- Document type: Application Guide
- Page count: 2
- Read time: 5 minutes
Key Takeaways
- Prepare buffer as described in USP <129>
- Discover alternatives to serological pipettes
- Prevent Mycoplasma contamination and endotoxin interferences
- Sample safely in sterility testing canisters for deviation investigations
- Prevent false positive with the right selection of lab water and pipettes
This Resource is Designed for:
- Quality Control
- Method Development
- Analytical Excellence
- Quality Assurance
- Cell Culture
- Microbiology
Applications Supported:
- Microbial Enumeration
- Cell Cultivation
- Buffer and Media Preparation
- Endotoxin Testing
- Mycoplasma Retention