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SoloHill® Microcarriers

Animal Protein Coated and Animal Product Free Microcarriers

SoloHill® Microcarriers promote excellent anchorage-dependent cell line attachment and growth and have been used for the production of animal and human health products for over 30 years. Without hydration or pre-swelling needed, microcarriers are easily sterilized for use, and their solid core design simplifies seed train preparation. Available both in animal protein-coated and animal product free, SoloHill® Microcarriers fit into any process and are an excellent choice for scale-up projects where process development timelines and capital investment are limiting.

Find the Right Solution

Animal Product Free

SoloHill® Animal Product Free Microcarriers are for researchers working in limited or without animal sources in their processes. Sartorius offers a high performing certified animal-free microcarrier, perfect for researchers who desire to work within a completely serum-free media and animal product free (APF) process.

Attributes Hillex® Plastic Plastic Plus Star-Plus
Description Modified Polystyrene Cross-linked Polystyrene Cross-linked Polystyrene Cross-linked Polystyrene
Size Range 160 – 200 µm 90 – 150 & 125 – 212 µm 125 – 212 µm 125 – 212 µm
Specific Gravity 1.090 – 1.150 1.022 – 1.030 & 1.034 – 1.046 1.022 – 1.030 1.020 – 1.035
Area/Gram 515 cm2 480 & 360 cm2 360 cm2 360 cm2
Surface Charge Yes No Yes Yes
Animal Protein Coated
Collagen Coated FACT III (Collagen)
Description Cross-linked Polystyrene Cross-linked Polystyrene
Animal Protein Yes – Type 1 porcine collagen coating Yes – Surface modified Type 1 porcine collagen coating
Size Range 90 – 150 & 125 – 212 µm 125 – 212 µm
Specific Gravity 1.022 – 1.030 & 1.034 – 1.046 1.022 – 1.030
Area/Gram 480 & 360 cm 360 cm2
Surface Charge No Yes
Applications

SoloHill® Microcarrier Applications

Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers. Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.

Researcher Reported Applications

Cell Line Description Star Plus Plastic & Plastic Plus Collagen Coated Charged Collagen Coated
VERO monkey kidney x x x
CEF chick embryo fibroblast x
ST swine testes x
BT bovine turbinate x
P4RSF rabbit skin fibroblast x
MRC-5 human diploid fibroblast x
KB human epithelial carcinoma x
HFF human foreskin fibroblast x x x
UMSCC human squamous carcinoma x x x x
MDBK Madin-Darby bovine kidney x x
CHO chinese hamster ovary x
MDCK Madin-Darby canine kidney x
hMSC Human Mesenchymal Stem Cells x x

SoloHill® Microcarriers for Therapeutics and Vaccines

SoloHill® microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.

FDA-licensed human therapeutics:

  • Thyroid cancer diagnostic
  • Thyroid cancer therapy
  • Beta interferon – MS indication

USDA-licensed animal vaccines:

  • Equine influenza
  • Canine parvovirus
  • Feline leukemia and many others

SoloHill scientists are actively researching new applications. This research has led to completed development on novel human vaccine protocols.

  • Human influenza vaccine
  • Marek’s disease vaccine

Animal Product Free Microcarriers

Hillex®

The cationic amine-modified microporous surface of the Hillex® microcarriers with polystyrene core improves cell attraction and attachment without affecting cell harvest efficiency.

Description

The polystyrene core of the Hillex family of microcarriers is modified with a cationic amine which improves cell attraction. A unique microporous surface improves attachment without affecting harvest efficiency.

  • Size range: 160-200 microns
  • Specific gravity: 1.09-1.15
  • May be sterilized by autoclaving with appropriate end user validation, or by gamma irradiation

Animal Product Free Starter Kits

The best microcarrier and attachment conditions may vary between cell types; experimentation will allow the most confident selection of the optimal microcarrier. To accelerate your evaluation process, SoloHill microcarriers are available in a convenient starter kit.

The SoloHill Microcarrier Starter Kit makes it easy to design multivariate experiments which explore the performance of SoloHill microcarriers across media formulations and cell lines. This Animal Product Free kit has been designed for general cell culture use with any anchorage-dependent cell line.

Each kit contains: 5 grams each of: Hillex II, Plastic, Plastic Plus and Star-Plus microcarriers.

Packaging Formats

SoloHill microcarriers are now available in two product packaging formats:

  • Stocked bottle format is suitable for research and process development applications. Microcarriers in this format are delivered non-sterile and bottled in convenient fixed weight quantities: 10 gm, 100 gm, 500 gm, and 1,000 gm.
  • For manufacturing use, microcarriers are packaged in the Allegro™ Microcarrier Delivery System (AMDS). The AMDS is a single-use package for aseptic delivery of sterile microcarriers (sterilized by gamma irradiation) directly to single-use or conventional fixed bioreactor vessels. AMDS delivers customer specific quantities from 30 grams to 8 Kilograms, ready for immediate use. For more information about this format, contact ​Sartorius below.

Technical Support

SoloHill microcarriers are the most complete line of Animal Product Free microcarriers available to address regulatory matters and today’s market requirements. Sartorius’ technical support specialists can assist you in identifying the optimal microcarrier for your needs as well as provide example protocols to suit many process requirements.

Contacting technical support connects you to a worldwide network of microbiologists and cell culture scientists who are actively engaged in hands-on research and development. They can provide consultation on protocol development, microcarrier selection and industry trends. For those seeking more comprehensive support, training services are available focusing on microcarrier selection, process optimization, and more. Our support ensures experienced researchers and those using microcarriers for the first time are employing state of the art approaches and maximizing resources.

Specifications

Series and Description Model # Relative
Density
Diameter
(μm)
Area/Gram
(cm2)
Surface
Charge
Drug
Master File
Hillex®II
modified polystyrene
H112 – 170 1.090-1.150 160-200 515 x MF10059

Total Quality System certified to ISO 9001:current standards

Ordering Information

Series Model # Catalog # Weight
Hillex®II H112 – 170 H-170-020 10g
H-170-050 100g
H-170-070 500g
H-170-080 1000g

Animal Product Free (APF) Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
Hillex II H112-170 AMDS05HS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS

Plastic

Compared to porous microcarriers, styrene copolymer microcarriers are constructed from solid non-toxic plastic which prevents absorption of toxic byproducts and makes harvesting simple with cell viabilities typically greater than 95%. The plastic beads allow for easy process transfer out of traditional styrene labware methods such as roller bottles and monolayers.

Description

Specifications

Ordering Information

Series Model # Catalog # Weight
Plastic P102-915 P-215-070 500g
P-215-080 1000g
P102-1521 P-221-020 10g
P-221-050 100g
P-221-070 500g
P-221-080 1000g
P104-1521 P-421-070 500g
P-421-080 1000g

Animal Product Free (APF) Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
Plastic P102-1521 AMDS05PS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS

Plastic Plus

Incorporated with an electric charge, the Plastic Plus microcarriers strengthen cell attraction and attachment. Like the non-toxic Plastic microcarriers, Plastic Plus are solidly constructed from styrene copolymer, and harvest cell viabilities are typically greater than 95%. 

Description

Specifications

Ordering Information

Series Model # Catalog # Weight
 Plastic Plus PP102-1521 PP-221-020 10g
PP-221-050 100g
PP-221-070 500g
PP-221-080 1000g

Animal Product Free (APF) Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
Plastic Plus PP102-1521 AMDS05PPS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS

Star-Plus

The Star-Plus microcarriers are a positive-charge, rigid spherical Animal Protein Free microcarrier and under a variety of environmental conditions, foster quick cell attachment and excellent growth of multiple cell types. Ideal for the incorporation into single-use manufacturing processes, each manufactured lot of Star-Plus microcarriers is performance tested for cell attachment and growth.

Description

Specifications

Ordering Information

Series Model # Catalog # Weight
Star-Plus SP102-1521 SP-221-020 10g
SP-221-050 100g
SP-221-070 500g
SP-221-080 1000g

Animal Product Free (APF) Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
Star-Plus SP102-1521 AMDS05SPS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS

Animal Protein Coated Microcarriers

Collagen Coated

For anchorage-dependent cell lines, collagen has demonstrated to be an effective promoter of cell attachment and growth. SoloHill® Collagen Coated microcarriers promote rapid attachment and cell growth for a large variety of cell lines.  

Description

Specifications

Ordering Information

Series Model # Catalog # Weight
Collagen Coated C102-915 C-215-070 500g
C-215-080 1000g
C102-1521 C-221-020 10g
C-221-050 100g
C-221-070 500g
C-221-080 1000g
C104-1521 C-421-070  500g
C-421-080 1000g

Standard Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
Collagen Coated C102-1521 AMDS05CS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS.

FACT III

Compared to standard collagen-coated microcarriers, Fast Attachment Collagen Treated (FACT) have improved attachment kinetics and enhance growth performance through the utilization of proprietary surface modification of the collagen layer.

Description

Specifications

Ordering Information

Series Model # Catalog # Weight
FACT III F102-152 F-221-020 10g
F-221-050 100g
F-221-070 500g
F-221-080 1000g

Standard Starter Kit

Allegro™ Microcarrier Delivery System: Gamma Irradiated Evaluation Kits**

Series Model # Catalog # Weight
FACT III Coated F102-1521 AMDS05FS100 100g

** AMDS kits for evaluation are delivered sterilized by gamma irradiation ( < 25 kGy). For additional technical information about the AMDS or to order specific quantities from 30 grams to 8 kilograms per AMDS

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