Biostat® RM Top Features
Discover the Biostat® RM
Innovative Technology for Your Process Needs
Biostat® RM 20 | 50 basic is a perfectly sized, single-use, wave-mixed benchtop bioreactor for stand-alone use. It features an exchangeable bag holder to fit bags with a total volume of 1 – 50 L. The Biostat® RM Basic rocking platform with an integrated local controller, Air | CO2 mixing module and optional load cells is the optimal choice for straight-forward applications, such as seed generation.
The Biostat® B is our universal benchtop controller for stirred and rocking motion systems. It is available with the RM Rocker for advanced process control featuring pH, DO and BioPAT® Viamass. The system is fully configurable and can therefore serve various process needs, from process development to small scale commercial production.
Biostat® B with RM 200 Rocker is your choice for production-scale seed expansion and rapid material supply for preclinical and clinical studies using proven rocking motion technology. The bag holder fits the Flexsafe® RM bags in a working volume range from 10 up to 100 L.
The wave generated in the RM bags due to the rocking motion ensures effective gas exchange through the gas-liquid interface. Four different gas lines for air, O2, N2 and CO2 are equipped with flow meters and four high-precision mass flow controllers. An integrated pressure sensor continuously measures the pressure inside the bag. To ensure process safety, aeration is switched off when an upper threshold is reached.
The Flexsafe® RM bags are available in 1 L, 2 L, 10 L, 20 L, 50 L, 100 L and 200 L size. Bags are available in basic, optical and in two different perfusion configurations.
Basic bags are designed for use in seed train and production applications without pH and DO control. Optical bags feature integrated single-use opto-chemical pH and DO sensors, which are already pre-calibrated. Used together with the Biostat® RM control tower, they allow high end cultivation with full process automation. Basic and optical bags up to 50 L size are also available with screw caps (38 mm diameter). The screw cap can be used for the insertion | removal of larger objects inside a laminar flow cabinet, e.g. microcarriers. Perfusion bags are available in two versions: A) With an integrated 1.2 μm perfusion membrane (PES). The membrane is fixed at the bottom of the bag, forming a compartment for removal of cell free media. The wave created by the agitation moves over the surface of the membrane, thereby flushing the membrane with every rocking motion. This innovative and patented design ensures low fouling and long cultivation times. B) ATF perfusion bags are designed for the convenient connection to an external cell retention device via OPTA connector.
Flexsafe® RM Bags are supplied gamma irradiated and ready to use. The Flexsafe® RM bag is mounted on the bag holder of the rocker easily and secured on both sides through fixation clamps.
Precise and reliable single-use sensors for pH and DO come pre-installed and sterilized with the bag. The sensors are pre-calibrated and the calibration data is supplied together with the bag (for pH and DO). 10 to 200 L bags are also available with integrated sensors for measurement of viable biomass (BioPAT® Viamass). All 2nd generation Biostat® B with RM systems can be equipped with the BioPAT® Viamass electronics.
BioPAT® Viamass – viable biomass measurement
BioPAT® MFCS – Software for reliable data acquisition, monitoring and control