Pre-Packed Chromatography Columns

Rapid screening of chromatographic conditions is necessary to identify the best purification conditions. Scouting of conditions can be achieved on lab-scale chromatography systems or automated robotic systems. Especially novel functionalities like mixed-mode chromatographic materials require robust screening tools for process development. 

 

Find the Right Solution for Your Chromatography Process

PRC Pre-Packed Columns and Robo Columns for Screening are robust tools for quick purification development. Both products come pre-packed with ion exchange, mixed-mode, and affinity chromatographic materials.
Attributes  Robo Columns for Screening PRC Pre-Packed Columns
Column volumes  200 and 600 µL  1 and 5 mL 
Bed height  10 and 30 mm  50 and 100 mm 
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Robo Columns for Screening

Robo Columns for Screening are supplied in strips of 8 pre-packed units. The 200 µL units are suited for screening purification conditions and selecting chromatographic materials. The 600 µL units are recommended when longer residence times above 4 min are needed (e.g., evaluation of dynamic binding capacities).

Description

Applications

ScreenExpert RoboColumns are suitable for a large variety of applications such as:

  • Parallel screening of chromatography sorbents
  • Parallel screening and optimization of chromatographic conditions in downstream process development (conductivity, pH)
  • Scale down experimental work
  • Determination of dynamic binding capacity
  • Determination of the optimal residence time
  • Optimization of the regeneration step

Specifications

ScreenExpert RoboColumns
Column volume 200 μL 600 μL
Bed height 10 mm 30 mm
Column inner diameter 5 mm
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1 to 14
Avoid Halogenated organic solvents, hexane
Storage solution 20% ethanol /1 M NaCl
Recommended storage temperature 2 to 8 °C
Working temperature 4 to 30 °C
Maximum working pressure Up to 8.0 bar g (116 psi g)
Flow rate 16 to 1,000 cm/hr 1
Laser label Placed on every individual column body, and containing:

  • Product number
  • Sorbent name
  • Column volume in μL
96-Well Array Plate
Size 128.3 x 86.0 x 14.0 mm
Material Polyoxymethylene copolymer
Number of wells 96

1 The Liquid Transfer menu of the Te-Chrom Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te-Chrom Wizard Software Manual, 396076, en, V1.0).

Ordering Information

Description Part Number
ScreenExpert RoboColumn MEP HyperCel 200 μL, row of 8 SR2MEP
ScreenExpert RoboColumn MEP HyperCel 600 μL, row of 8 SR6MEP
ScreenExpert RoboColumn HEA HyperCel 200 μL, row of 8 SR2HEA
ScreenExpert RoboColumn HEA HyperCel 600 μL, row of 8 SR6HEA
ScreenExpert RoboColumn PPA HyperCel 200 μL, row of 8 SR2PPA
ScreenExpert RoboColumn PPA HyperCel 600 μL, row of 8 SR6PPA
ScreenExpert RoboColumn HyperCel STAR AX 200 μL, row of 8 SR2STARAX
ScreenExpert RoboColumn HyperCel STAR AX 600 μL, row of 8 SR6STARAX
ScreenExpert RoboColumn CM Ceramic HyperD F 200 μL, row of 8 SR2CMCHDF
ScreenExpert RoboColumn CM Ceramic HyperD F 600 μL, row of 8 SR6CMCHDF
ScreenExpert RoboColumn KANEKA KanCapA♦ 200 μL, row of 8 SR2KANCAPA
ScreenExpert RoboColumn KANEKA KanCapA 600 μL, row of 8 SR6KANCAPA
ScreenExpert RoboColumn CMM HyperCel 200 µL, row of 8 SR2CMM
ScreenExpert RoboColumn CMM HyperCel 600 µL, row of 8 SR6CMM
96-well RoboColumn array plate SR96WAP
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Multi-Well Plates (empty)

Description Part Number
AcroPrep Advance 96-Well Filter Plate, 1 mL, 0.45 μm Supor® membrane (5/pkg) 8129
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AcroPrep Advance 96-Well Filter Plate for Ultrafiltration

Description Part Number
350 μL, Omega™ 3K MWCO (10/pkg) 8033
350 μL, Omega 10K MWCO (10/pkg) 8034
350 μL, Omega 30K MWCO (10/pkg) 8035
350 μL, Omega 100K MWCO (10/pkg) 8036
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PRC Prepacked Columns

Description Part Number
PRC Column 5×50 MEP HyperCel, 1 mL PRC05X050MEPHCEL01
PRC Column 8×100 MEP HyperCel, 5 mL PRC08X100MEPHCEL01
PRC Column 5×50 HEA HyperCel, 1 mL PRC05X050HEAHCEL01
PRC Column 8×100 HEA HyperCel, 5 mL PRC08X100HEAHCEL01
PRC Column 5×50 PPA HyperCel, 1 mL PRC05X050PPAHCEL01
PRC Column 8×100 PPA HyperCel, 5 mL PRC08X100PPAHCEL01
PRC Column 5×50 HyperCel STAR AX, 1 mL PRCSTARAX1ML
PRC Column 8×100 HyperCel STAR AX, 5 mL PRCSTARAX5ML
PRC Column 5×50 Q Ceramic HyperD F, 1 mL PRC05X050QCHDF01
PRC Column 5×50 CM Ceramic HyperD F, 1 mL PRC05X050CMCHDF01
PRC Column 5×50 KANEKA KanCapA, 1 mL PRC05X050KANCAPA
PRC Column 8×100 KANEKA KanCapA, 5 mL PRC08X100KANCAPA
PRC Column 5×50 CMM HyperCel, 1 mL PRCCMMHCEL1ML
PRC Column 8×100 CMM HyperCel, 5 mL PRCCMMHCEL5ML
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*KANEKA KanCapA is a trademark of Kaneka Corporation

PRC Pre-Packed Columns

PRC Pre-Packed Columns come in 1 and 5 mL formats ready to use. The 1 mL columns are recommended for scouting purification conditions, which can be verified and further optimized in the 5 mL format with 10 cm bed height. 

PRC Prepacked Chromatography Columns with HyperCel™ STAR AX…

PRC Prepacked Chromatography Columns with HyperCel™ STAR AX Salt-Tolerant Anion Exchange Sorbent

HyperCel™ STAR AX sorbent is composed of a rigid cellulose matrix that has excellent flow properties and generate low backpressure. Available in convenient prepacked chromatography columns, this product is ideally suited for rapid screening and condition optimization. Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Description

Rapid selectivity screening of HyperCel STAR AX anion exchanger.

  • Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems.
  • Efficient – High packing efficiency (≥ 2,500 plates/meter).
  • Consistent – Screen HyperCel STAR AX sorbent under reliable and reproducible conditions, and guarantee performance run after run.

“Salt-Tolerant” Anion Exchange Chromatography Sorbent

HyperCel STAR AX sorbent is composed of a rigid cellulose matrix that has excellent flow properties and generates low backpressure. It provides:

  • High dynamic binding capacity at short residence time
  • Use in either capture, intermediate or polishing steps
  • High dynamic binding capacity is maintained up to 15 mS/cm conductivity
  • Direct scale up to pilot or production scale columns

Applications

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate separation conditions. Once the separation conditions are selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.

HyperCel STAR AX Anion Exchange Chromatography Sorbents

  • Native and recombinant proteins
  • Vaccines
  • Monoclonal and polyclonal antibodies
  • Plasma derivatives
  • Biopharmaceuticals

Specifications

Sorbent
Volume 1 mL (5 mm I.D. x 50 mm) or 5 mL (8 mm I.D. x 100 mm)
Average particle size 60 – 90 µm
Dynamic binding capacity1 >100 mg BSA/mL within pH range 7.5 – 8.0 (conductivity 2 – 15 mS/cm)
Ligand Primary amine
Storage solution 30% isopropanol, 100 mM sodium phosphate, pH 4.3
Working pH Binding: pH 7.0 – 8.5; Elution: pH 3.0 – 8.5
Cleaning pH 1 – 14
Working pressure2 < 0.5 bar (7 psi) for 1 mL column; < 1 bar (14 psi) for 5 mL column
Column
Outer dimensions 10 x 100 mm for 1 mL column or 11.5 x 140 mm for 5 mL column
Materials of construction Body and end caps: Molded PP (Polypropylene)
17 µm frit: PP / PE (Polypropylene / Polyethylene)
Connections3 Built-in 10-32 fittings

1 Determined at 10% breakthrough, using 5 mg/mL bovine serum albumin (BSA) in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time (RT). Conductivity 2 – 15 mS/cm by supplementation of NaCl.

2 Pressure at 600 cm/hr equivalent to: 2 mL/min for 1 mL column, 5 mL/min for 5 mL column, in 0.1 M NaCl.

Ordering Information

HyperCel STAR AX PRC Prepacked Columns
Description Part Number
PRC Column 5×50 HyperCel STAR AX, 1 mL PRCSTARAX1ML
PRC Column 8×100 HyperCel STAR AX, 5 mL PRCSTARAX5ML
HyperCel STAR AX Sorbent
Size Part Number
5 mL 20197-018
25 mL 20197-026
100 mL 20197-032
1 L 20197-046
5 L 20197-058
10 L 20197-064

PRC Prepacked Chromatography Columns with MEP, HEA, and PPA…

MEP, HEA and PPA HyperCel™ sorbents exploit unique selectivity of robust synthetic ligands to capture proteins or separate them from contaminants. They provide a unique separation mechanism different from conventional methods. All ligands operate predominantly by hydrophobic interaction. This “HIC-like” interaction typically takes place without the addition of lyotropic salt. This prepacked column format conveniently enables rapid screening and condition optimization. 

Description

Applications

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction

  • MEP HyperCel sorbent: Antibody capture; alternative to conventional hydrophobic interaction
  • MEP, HEA, PPA HyperCel sorbents: No salt/low salt alternative to hydrophobic interaction

Specifications

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)
5 mL (8 mm ID x 100 mm)

Functionalities
Mixed-mode: MEP, HEA and PPA HyperCel sorbents

Storage Solution
30% isopropanol/100 mM sodium phosphate, pH 4.3

Working Pressure1
< 0.5 barg (7 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns
11.5 x 140 mm for 5 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns
30 barg (435 psig) for 5 mL columns

1 Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

Mixed-mode Sorbent Ligand Average Particle Size (µm) Ligand Density (µmole/mL) Dynamic Capacity (mg/mL)1
MEP HyperCel 4-mercapto-ethyl-pyridine (pKa = 4.8) 90 80 – 125 > 202
HEA HyperCel Hexylamine (aliphatic) (pKa = 8.0) 90 58 – 84 > 403
 PPA HyperCel Phenylpropylamine (aromatic) (pKa = 8.0) 90 58 – 80 > 403

1 Determined at 10% breakthrough using:
2 5 mg/mL human IgG in PBS, flow rate 60 cm/h
3 5 mg/mL BSA in PBS, flow rate 100 cm/h

Additional Information

Sartorius offers a comprehensive line of chromatography columns from lab to process scale.

PRC Columns
1 – 5 mL (prepacked)
Fast selectivity screening

  • With Q and CM Ceramic HyperD Ion Exchange Sorbents
  • With MEP, HEA, and PPA HyperCel Mixed-mode Sorbents
Resolute® Columns
6 – 300 L
Pilot and small-scale production
Resolute® Columns
50 – 1500 L
Large-scale production

Ordering Information

PRC Prepacked Sorbent Ordering Information

Part Number Description Packaging
PRC05X050MEPHCEL01 PRC Column 5×50 MEP HyperCel 1 mL
PRC05X050HEAHCEL01 PRC Column 5×50 HEA HyperCel 1 mL
PRC05X050PPAHCEL01 PRC Column 5×50 PPA HyperCel 1 mL
PRC08X100MEPHCEL01 PRC Column 8×100 MEP HyperCel 5 mL
PRC08X100HEAHCEL01 PRC Column 8×100 HEA HyperCel 5 mL
PRC08X100PPAHCEL01 PRC Column 8×100 PPA HyperCel 5 mL

PRC Prepacked Chromatography Columns with Q and CM Ceramic…

Q and CM Ceramic HyperD™ sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead.  Available in convenient prepacked chromatography columns, this product is ideally suited for rapid screening and condition optimization.

Description

Rapid selectivity screening of Ceramic HyperD ion exchangers

  • Convenient – Ready-to-use 1 mL prepacked columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems.
  • Efficient – High packing efficiency (> 2,500 plates/meter).
  • Consistent – Screen Pall ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Ion Exchange Chromatography Sorbents

Q and CM Ceramic HyperD sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. Typical features include:

  • Good dynamic binding capacity independent of flow rate
  • Allowing a direct capture with high binding capacity for IgG at conductivities of 10 to 15 mS/cm on CM Ceramic HyperD sorbent

PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

Applications

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Ion Exchange Chromatography Sorbents

  • Native and recombinant proteins
  • Plasmids
  • Vaccines
  • Monoclonal and polyclonal antibodies
  • Plasma derivatives
  • Biopharmaceuticals

Specifications

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)

Functionalities
Ion exchange: Q and CM Ceramic HyperD F sorbents

Storage Solution
20% ethanol/150 mM NaCl

Working Pressure1
< 1.5 barg (22 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns

Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
 

Sorbent Average Particle Size (µm) Ionizable Groups (µEq/mL) Dynamic Capacity (mg/mL)1
Ion Exchange Chemistry 
Q Ceramic HyperD F Quaternary amine 50 > 250 > 852
CM Ceramic HyperD F Carboxymethyl 50 > 250 > 603

1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h
3 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h

Ordering Information

Part Number Description Volume
PRC05X050QCHDF01 PRC Column 5×50 Q Ceramic HyperD F 1 mL
PRC05X050CMCHDF01 PRC Column 5×50 CM Ceramic HyperD F 1 mL