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Large Molecule Characterization

The 8-channel Octet QKe System Provides Rapid Characterization of Antibodies, Proteins, and Other Large Biomolecules (> 5000 Da)

Monitor binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants(KD).  Screen kinetics of 64 samples in 1.5 hours.Analyze samples in crude matrix – The fluidics-free Dip and Read technology with samples in a plate format combined with ready-to-use biosensors allows for high flexibility in sample type. Quantitation of 96 samples can be accomplished in 32 minutes.

Applications

Kinetic and Affinity Characterization

Octet instruments accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.

Protein Quantitation in Bioprocessing

Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA.

Technology Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Sample volume 180–220 µL/well
Nondestructive testing, easily recovered
Data presentation Sensorgrams displaying kinetic traces or concentration binding rates
Epitope binning and cross-blocking matrices and trace overlays
Tabulated kinetic or concentration data
Automation Up to 8 assays in parallel; up to 96 assays per 96-well plate
Orbital flow capacity Static or 100–1500 rpm
Analysis temperature Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka) 102 to 105  M-1 s-1
Dissociation rate constant (kd) 10-5 to 10-2  s-1
Affinity constant (KD) 0.1 mM to 0.1 nM
Baseline noise (RMS) ≤ 8 pm
Baseline drift < 0.1 nm/hour
Quantitation range 1 µg/mL to 700 µg/mL at low rpm, LOD of 0.05 µg/mL at 1000 rpm
Quantitation precision ≤10%
Weight 54 lb (24.5 kg)
Dimensions (H x W x D) 18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)
Sample type Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Sample plate Standard, 96-well, black, flat-bottom microplate
Molecular weight detection >5000 Da

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