TFF Cassettes
Efficient filtration is a critical step in biopharmaceutical processing. Sartorius tangential flow filtration (TFF) cassettes provide high-performance separation in various upstream and downstream bioprocessing unit operations. They are available in a range of molecular weight cut-offs and micron range, from lab scale to process scale, in single-use and re-usable formats. When used together with associated TFF systems and filter holders, these TFF cassettes provide a complete seamless solution for efficient, robust, and reproducible TFF operations, including ultra- and microfiltration.
Find the Right Tangential Flow Filtration Cassette
For the retention of materials in the ultra-scale range (e.g., proteins, mAbs, or viral particles)
Attribute | Hydrosart® | Polyethersulfone (PES) |
|---|---|---|
Nominal Molecular Weight Cut-offs | 2, 5, 10, 30, 100, 300 kDa | 1, 5, 8, 10, 30, 50, 100, 300 kDa |
Filtration Areas | 10 cm², 50 cm², 200 cm², 0.1 m², 0.4 m², 0.6 m², 0.7 m², 1.4 m², 3.0 m², 3.5 m², 7 m², 14 m² | 10 cm², 50 cm², 200 cm², 0.1 m², 0.4 m², 0.6 m², 0.7 m², 1.4 m², 3.0 m², 3.5 m² |
Typical Process Fit | Peptide, Oligonucleotide, Proteins (Albumin), Blood Factors, Monoclonal antibodies (mAb), Vaccines, Cell & Gene Therapy, pDNA, mRNA, ADC, recombinant proteins, Viral vectors | Proteins (Albumin), Blood Factors, Monoclonal antibodies (mAb), Vaccines, Cell & Gene Therapy, pDNA, mRNA, ADC, recombinant proteins, Viral vectors |
Self-contained | Available | Partially Available |
For the retention of material on the micro-scale range (e.g., cells and visible particles)
Attribute | Hydrosart® | Polyethersulfone (PES) |
|---|---|---|
Pore Size | 0.2 & 0.45 µm | 0.1 µm |
Membrane Areas | 200 cm², 0.1 m², 0.4 m², 0.6 m², 3.0 m² | 200 cm², 0.1 m², 0.6 m², 3.0 m² |
Typical Process Fit | Cells, Particles | Cells, Particles, Mycoplasma reduction |
- Pre-flushed, sterile, ready to use.
- No cleaning or "extended" water flush is required, which saves time on setup and validation.
- High product yield and high sustained flux due to low-protein-binding membrane.
- No glues used, resulting in low extractables|leachables
Membrane Materials
Hydrosart®
Hydrosart® high-performance membrane is a stabilized cellulose-based membrane that has been optimized for biopharmaceutical process applications.
- Stable across a broad pH range.
- Extremely hydrophilic, making it non-protein binding and virtually non-fouling.
- Membrane cleaning, storage and depyrogenation can be accomplished by using NaOH even at elevated temperatures and concentrations.
Polyethersulfone
The polyethersulfone (PES) membrane is well-established in the biotechnological and pharmaceutical industries.
- Stable polymer that features a broad pH and temperature range.
- Membrane regeneration, storage and depyrogenation can be accomplished by using NaOH even at elevated temperatures.
- Ideally suited for biotechnology applications
Product Portfolio
Sartocon® and Sartocube® Microfiltration Cassettes
Sartocon® and Sartocube® Microfiltration cassettes are available with effective filtration areas from 0.02 m² up to 3.0 m². Available pore sizes are 0.45 µm and 0.2 µm with Hydrosart® membrane as well as 0.1 µm with polyethersulfone membrane .
Sartocon® and Sartocube® Ultrafiltration Cassettes
Sartocon® and Sartocube® Ultrafiltration cassettes are available with Hydrosart® and polyethersulfone membrane with effective filtration areas from 50 cm² up to 3.5 m². Available cutoffs range from 1 kDa up to 300 kDa.
Single-Use Sartocon®️ and Sartocube®️ Self-Contained Units
Sartocon®️ and Sartocube®️ Self Contained Units (SCUs) are 0.14 m² to 14 m² pre-sterilized disposable units suitable for cGMP environments. The SCU is available in a variety of molecular weight cut-offs and in Hydrosart®️ and polyethersulfone (PES) membrane types.
Sartocon®️ SP | Single pass TFF Cassettes
Single-pass TFF simplifies ultra- and diafiltration by simultaneously concentrating and diafiltration in one pass instead of multiple recirculations.
The Sartocon® SP cassette is a self-contained closed processing device that is delivered irradiated to minimize biocontamination risk.
