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Plasmid Engineering and Sequencing Services
Plasmids are DNA vectors that remain critical material for the development of a wide range of advanced biotherapies: gene therapy (AAV, LV), gene-modified cell therapy (CAR-T), mRNA-based therapies and more. Plasmids control the expression of genes of interest to be transferred in a biological system for research, bio-processing or therapeutic application. By optimizing plasmid design, researchers and developers can enhance the safety, efficacy, and specificity of biotherapies, ultimately improving treatment outcomes. Accessible through our intuitive online platform, plasmid engineering is powered by our unique and innovative DNA assembly technology (e-Zyvec®). Thanks to its flexibility and accuracy, our technology simplifies the generation of any type of plasmid, from the easiest to the most sophisticated. Tailor-designed plasmids are also easily transferable to our plasmid manufacturing processes to obtain higher quantities of higher grade (up to GMP) materials.
Sartorius offers a modular, user-friendly platform for designing custom plasmids, with expert support and technology that ensures precise assembly—even for complex or novel constructs. For simpler needs, we provide pre-designed plasmids through a fast, cost-effective service that includes sequencing and discovery-grade pDNA preparation. To guarantee accuracy, all engineered plasmids undergo thorough validation using in-house Oxford Nanopore-based NGS platform, which is also available for customer DNA analysis.
We offer an innovative modular approach to plasmid design, applicable to any type of plasmid. With our user-friendly plasmid platform, you can freely design your plasmids, focusing on your sequences of interest. Our experts will then review your design and advise you, when required, about relevant ways to improve your molecules.The e-Zyvec® technology ensures that each plasmid assembled accurately contains all your sequences, including repeated sequences, high or low GC contents, long sequences, or multiple transgenes. If the plasmid you need does not exist yet, we can make it for you.
Design My Plasmids
For the most basic needs, we also offer a range of simple plasmids, available on our plasmid-services platform . Pre-designed plasmids are provided at competitive costs and shortened turn-over times.
What’s included in this offer?
Explore Easy Plasmid
Small sequence modifications, such as a single point mutation, can impair the biological functionality of a plasmid, thus jeopardizing downstream prcesses. To mitigate this risk as much as possible, we have developed our own Next Generation Sequencing analytical platform based on ONT, so all our engineered plasmids can be delivered with a comprehensive sequencing report..This capacity is also made accessible to our customer for NGS validation of their own DNA molecules. Our bioinformatics combine long-read mapping and de novo assembly applicable to either circular or linear DNA molecule.
Personalized studies using either ONT or Illumina technologies are also available through service contract. Please inquire.
Create Your Account Graphical representation of the sequencing data across the entire reference sequence of the sample, highlighting potential mutations and sequencing depth.
Designed as a one-stop-shop for plasmid services, our free of access online design platform is simply accessible from your MySartorius account. With our intutive plasmid configurator, you will be able to start new projects for plasmid engineering, sequencing, and more. This is the most direct way to get in contact with our project management experts and request quotes for our plasmid services.Our pDNA Services:
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We deliver “discovery grade*” plasmids as circular, double stranded DNA molecules, in a screw-cap plastic tube, re-suspended in 1X AE buffer (miniprep.) or 1X TE buffer (midi, maxi, giga prep.), at room temperature. Please note that we DO NOT provide bacterial stock.
Discovery grade plasmids can be ordered at multiple formats, please inquire for more information.
For larger scale or higher quality grade plasmid production please inquire Plasmid Manufacturing Service
*Discovery grade means that while plasmids are produced in a sterile environment according to good laboratory practice, quality control are limited to basic quality and identity test. Discovery grade plasmids should be restricted to Research Application.
Upon receipt, briefly spin down the tubes containing your engineered plasmids (bench centrifuge) and store them at:
We strongly recommend to aliquot the Polyplus discovery grade plasmids for long storage and to avoid freeze-thaw cycles.
Duration of storage are given here as indication. You should always control the integrity of long stored plasmids by running gel electrophoresis (denaturation) or sequencing (sequence integrity).
We do offer a pDNA amplification service for either engineered plasmids we generated, or your own plasmid you may wish to send us.
Amplification can be performed at small scale (up to several mg) and discovery grade, or at large scale (up to several gram) at R&D, High Quality or GMP grade with our Plasmid Manufacturing Service.
You can send your samples following the guidelines below:
Samples should be sent to:
Polyplus-TransfectionProduction Team80 rue du Dr Yersin59120 LoosFRANCE
When known, please enter the sequences of reference of your samples on our platform.
After sequencing your sample, we will provide you the full-length sequence of your plasmid (.fasta file) as well as a sequencing report (.pdf file) containing:
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