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Boost Gene Therapy with pPLUS® Plasmids
Improve Your Viral Vector Process
Complementary to our transfection reagents and cell culture media for gene therapy, we have developed pPLUS® off-the-shelf packaging plasmids for AAV and LV production. These innovative plasmids enhance efficiency and productivity, providing a reliable solution for researchers and manufacturers. With optimized features, ready-to-use formats, and availability in Research, High Quality, and GMP grades, pPLUS® plasmids ensure high-quality results in gene therapy applications.
Effective gene therapy using AAV or LV vectors depends on careful plasmid design to improve vector yield, while ensuring regulatory compliance. Discover pPLUS® high-quality and optimized plasmids engineered to make your gene therapy a success.
pPLUS® AAV are a set of Helper and RepCap plasmids designed for AAV production of various serotypes. These plasmids are optimized to work together with FectoVIR®-AAV in a triple transfection process, providing enhanced AAV titers and infectivity. They are readily available from Research grade to GMP.
Discover pPLUS®AAV-Helper Discover pPLUS®AAV-RC Range
Elevate your lentivirus production with pPLUS® LV, a designed set of GAG/POL, REV, and VSV-G plasmids. Engineered to seamlessly integrate with FectoVIR®-LV in a third-generation transfection process, these plasmids deliver enhanced yields with high-quality titers. They are readily available in Research grade and can be custom made to HQ and GMP grade.
Discover pPLUS® LV
Select plasmids from off-the-shelf to custom-plasmid.
There is no minimum ordering quantity.
Sartorius recommends adjusting the plasmid ratios according to your production process. To determine the optimum transfection conditions, including pDNA ratios for rAAV or LV production, Sartorius recommends using a Design of Experiment (DoE). For plasmid ratios optimization, Sartorius recommends using a Mixture Design approach which is the most appropriate for this purpose.
Sartorius can provide support in building a DoE experimental plan and analyzing the results. If you would like a more complete DoE proposition adapted to your goals and constraints, feel free to contact support here.
pPLUS® are shipped in dry ice. Fee for the dry ice packaging will be charged to customers and included in the shipment cost. This will be automatically indicated in the quote, in the product description table.
Our plasmids are license and royalty-free, even though pPLUS® AAV-Helper is patented. Sartorius remains the sole provider, meaning that amplification of the plasmid cannot be performed with any manufacturer other than Sartorius. For more information, please refer to the terms and conditions of our products.
Novel engineered pHelper plasmid to improve yield and quality of several AAV serotypes in suspension cell culture systems.
This webinar showcases the optimization of a scalable LV production process using a high-throughput, automated microbioreactor&nbs...
Enhance transfection with a next-generation transfection reagent and helper plasmid.
Learn how AAV helper plasmid design enhances rAAV production yield and quality.
Join this webinar to understand raw material quality grades and requirements for ATMP manufacturing.
Discover our transfection reagents for various applications, designed to fit with our plasmids and enhance your productivity.
Discover our plasmid DNA manufacturing services, offering scalable solutions in state-of-the-art GMP facilities.
Utilize our e-Zyvec® assembly technology to design & optimize plasmid sequences tailored to your specific applications.
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