Translational Insights for Reproducible Advanced Cell Models
Cells are the building blocks of all organisms. Understanding the biology of cells and their interactions at the molecular level is the foundation of biomedical and life science research. Utilizing cells as models is an established approach for drug discovery, whereas their use as therapeutics is a rapidly growing approach.
More than 90% of therapeutic candidates fail to make it past phase III clinical trials, mainly due to their lack of efficacy. The use of 3D and stem cell-based models better recapitulate the pathobiology of diseases and offer in-depth predictive insights that can translate into positive clinical outcomes.
In recognition of this, the U.S. Food and Drug Administration (FDA) Modernization Act 2.0 allows scientists to now use cell-based models as alternatives to animal testing for drug applications.
Advanced cell models - such as organoids, spheroids and 2D stem cell-based models - are revolutionizing biomedical research and drug discovery. However, they come with several challenges:
싸토리우스 addresses these challenges and more with cutting-edge analytical solutions that streamline advanced cell model R&D workflows and provide translational insights that translate into clinical outcomes for patients in need.
Request a complimentary sample of our high-quality, animal-derived component-free Growth Factors and Cytokines for Research Use Only (RUO).
Request a complimentary sample of our ready-to-use 핵산전달감염 reagents for life science research involving most mammalian cell lines.
Customize your free sample pack of our high-quality cytokines, growth factors, media, and 핵산전달감염 reagents for optimal cell cultures.
3D cell models such as organoids and spheroids more closely recapitulate human cellular ecosystems which greatly improves clinical translation of new treatments.
The goal of 3D cell culture is to enable more physiologically relevant and translational models in a wide range of biomedical applications including disease modeling, drug discovery and 선별 as well as personalized medicine. Through comprehensive characterization, scientists can develop a deeper understanding of biology, ensure more robust maintenance of stem cells during extended cultures and monitor cells at every step of the 3D 오가노이드 and spheroid workflow.
What are Organoids and Spheroids?
Organoids are self-organizing, three-dimensional systems which retain many physiological characteristics of the native tissue from which they are derived. Accordingly, these miniaturized models have significant advantages over the use of traditional immortalized cell lines in providing accurate information on human disease modelling and can be used in the fields of drug 선별, disease modeling, personalized medicine and many others.
Spheroids are simpler structures and less differentiated but still aggregation of one or more cell types in 3D cell cultures.
Pluripotent stem cells (PSCs), with their unique ability to differentiate and specialize into all the cell types of the body (referred to as 다분화능), are the most promising starting cell type source for building organoids. With appropriate signal stimuli in 3D culture systems, pluripotent stem cells can readily develop into organoids.
Patient-derived cancer or healthy cells are increasingly used for 오가노이드 and spheroid generation due to their advantages compared with immortalized cell lines such us greater biological relevance. Gene editing technologies can be used to correct mutations in primary cells to study gene correction in patients.
Verification of cell source prior to use with and Platforms
Gene modification of cells
Rapid testing for , bacteria and fungi contaminations
Rapid Sterility Testing
The 3D cell culture process begins with seeding dissociated tissue-derived or stem cells into a 3D semi-solid 세포외기질 expanding these cells in 규명된 media.
Characterization of 오가노이드 formation using real-time kinetic measurements on the Incucyte® Live-Cell Analysis System provides the ability to objectively assess the impact of growth conditions and seeding densities and define the conditions necessary to maximize expansion.
Visualization and Monitoring of 3D growth -
with iQue® HTS Platform
Cell Culture Media, Growth Factors and Cytokines
Pipetting 3D Cell Cultures
Cells continue to differentiate and organize themselves into an organ-like structure in vitro. The Incucyte® Live-Cell Analysis System can be used to kinetically and objectively quantify multiple parameters to assess 오가노이드 growth and health during extended passaging.
Characterization of 표지자 expression in advanced cell models (ACM) with iQue® HTS Platform
Optimizing Advanced Cell Models iQue® Technical-Note
Assessment of 다분화능 and differentiation of iPSCs
Selection and transfer of individual organoids and embedding organoids into 96- or 384-well plates.
The CellCelector Automated Cell Selection and Retrieval Platform enables the automated scanning, detection and 게이팅 of complex 3D structures.
Clonal Stem Cell 채취
Further development and growth of organoids and spheroids enabling the formation of more complex structures. Specialty media, support the expansion, differentiation, and maturation of 3D cell cultures.
During extended cultures is important to monitor the formation and growth of 오가노이드 and spheroid cultures to maintain their health and desired characteristics. The offer optimized protocols for real-time analysis and monitoring of organoids and spheroids.
Creation of advanced cell-based assays for downstream analysis and specific applications for the research area of interest such us 3D disease modeling, drug discovery and compound 선별.
Assessing T-Cell Phenotype and Function in a Spheroid T-Cell Killing Assay
Maximizing 오가노이드 Insight Using Live-Cell Imaging and Analysis
핵산전달감염 Reagents and 플라스미드 DNA
Evaluation of Critical Quality Attributes (CQAs) i.e. 오가노이드/ spheroid size, sphericity, compactness, and viability is crucial to ensure the quality and reproducibility of the experiment, such us disease modeling, drug discovery and 선별.
Some parameters are tested throughout the culturing process including 오가노이드 health, counts and testing for microbial contaminations is recommended at several steps to ensure reliability and reproducibility of results.
Sophisticated, quantitative analytical solutions which provide multi-parametric assays such and platforms can greatly support the assessment of critical quality attributes.
Close the “bench to bedside” gap with innovative tools providing data and translational insights you can trust.
Reduce the risk of downstream failures when utilizing advanced cell models with our high-throughput instruments and automated solutions.
Explore leading solutions to accelerate progress from R&D, to Process Development and Manufacturing, safely and cost effectively.
Explore our portfolio of high-quality cell culture media, buffers, 핵산전달감염 reagents, growth factors, and cytokines for research applications.
Explore ready-to-use 3D human-derived microtissue models, human primary cells, high-quality glass bottom cell culture dishes and plates for superior imaging, as well as toxicology testing services for industries such as cosmetics, pharmaceutical, chemical, and other regulated industries.
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*NexaGel® hydrogel is a ready-to-use, versatile synthetic matrix system that closely replicates the human microenvironment, making it ideal for research involving a wide range of cell types. NexaGel® hydrogels feature optimized multi-functional ligands and concentration formulations, suitable for various applications, including 3D cell models, stem cell spheroids, and organoids.
Explore NexaGel® Hydrogels
*NexaGel® is a registered trademark of 싸토리우스 Bioanalytical Instruments, Inc. For details on the registrations please refer to our website
Our extensive range of ready-to-use 핵산전달감염 reagents is suitable for research involving most mammalian primary cells and cell lines. These best-in-class 핵산전달감염 products enable the analysis of protein functions, research into signaling pathways, genome editing, and efficient RNA interference for the 핵산전달감염 of guide RNA and Cas9 expression.
Explore 핵산전달감염 Reagents
Cytokines and growth factors are key immune system signaling molecules that regulate immune responses, inflammation, cell growth, and differentiation. Their research has advanced our understanding of immune-related diseases and led to the development of targeted therapies and vaccines.
싸토리우스 produces high-quality, recombinant cytokines and growth factors for research purposes, free from animal-derived components. These rigorously tested products ensure reliable results, consistent supply, and meet the highest purity and efficacy standards, with ISO9001-certified quality management.
Explore RUO Growth Factors & Cytokines
싸토리우스 ready-to-use media are designed to support the growth and maintenance of a variety of cells and cell lines while meeting the highest quality standards. Each lot is manufactured under a strictly controlled process according to a Product Master Record for lot-to-lot consistency.
Explore Classical Media
Transform your 3D cell model workflows with reliable image resolution using new confocal imaging! Choose both fluorescence-based and label-free assays to continuously monitor morphology, growth, and phenotypic changes in spheroids, organoids, stem-cell-derived models, and patient-derived 3D cultures, and gain physiologically relevant data without disrupting the culture. Additionally, the system is optimized for high-throughput workflows, making it ideal for large-scale applications such as:
Explore Incucyte® Live-Cell Analysis
The CellCelector Flex Platform is a fully automated cell imaging and 채취 system developed for 선별, selection and isolation of single cells, clusters, spheroids and organoids as well as single-cell clones and adherent colonies.
Explore Clonal Stem Cell 채취
The iQue® 5 High-Throughput 선별 Cytometer is the fastest solution for HTS applications, offering assay range with up to 27 channels, individual gain settings, and flexible workflows in 96- and 384-well formats.
Ideal for screens where cells are precious or 제한 in number, iQue® 5 is the fastest way to generate high-content data from small samples. The more powerful version of iQue Forecyt® software simplifies the entire process, with pre-규명된 templates and enhanced analytics tools, designed for complex datasets.
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Octet®️ Label-Free Bio Layer Interferometry (BLI) technology uses optical biosensors to measure protein-protein interactions in parallel, without the use of detection agents. This robust and fluidics-free approach enables fast, real-time characterization of expressed proteins, even in complex and unpurified samples.
Explore Label Free Detection
Testing cell cultures for microbial contamination on a regular basis ensures culture performance and parameters remain consistent and reproducible.
Microsart® Mycoplasma, Bacteria and Fungi qPCR kits offer rapid, reliable and easy-to-use solutions for microbial contamination control in compliance with international guidelines. Get results in just three hours!
Explore Microbial Testing
Explore solutions that streamline advanced cell model R&D workflows and provide translational insights.
Avoid failures in your pre-clinical / clinical pipeline with translational research tools which recapitulate closer the human environment enabling better predictive outcomes.
Understand complex biology with greater clarity by utilizing leading-edge technology for the imaging and 유세포분석 characterization of 2D immune and stem cells as well as complex, self-organizing 3D models.
Monitor continuously your 2D and 3D cell colonies, organoids and spheroids as they form and grow over time in controlled environment and capture quantitative information about their morphology, differentiation and health.
Reduce downstream risks with data you can trust – from the start.
Read about PSCs, their role in developing organoids, and the many emerging and exciting applications for organoids.
In order to effectively use 오가노이드 models specific and reliable culture and analysis methods are required; learn how.
Exploring the next generation of antibody therapeutics, such as antibody–drug conjugates (ADCs), bispecific antibodies and nanobodies.
Learn about the impact of proper pipetting on 3D cell cultures, and which techniques can improve the reproducibility of various experiments.
Measure T-cell response in 3D models with characterization reagents and validated spheroid washing and dissociation protocols.
Organoids are 규명된 by three key characteristics: self-organization, spatially restricted cell fate decisions, and ability to perform at least one function observed in organ tissue.
Spheroids are also 3D cell culture models, but they represent simple structures of typically the same cell types thus providing a versatile tool for translational research and disease modelling. There is a higher order self-assembly in organoids as opposed to spheroid cultures, and the former is more dependent on a matrix for its generation.
The 어플리케이션 with techniques initially developed for analysis with 2D cultures and 3D culture production and specification are seen as main challenges. Still 제한 by the technologies used to characterize these complex cell models and may introduce variability due to lack of environmental control and inadequate 3D Cell Culture protocols that compromise 오가노이드 or spheroid cell health.
The CellCelector Flex platform enables gentle 채취 of a wide range of 오가노이드 sizes, ranging from 80 μm to 3.5 mm with exceptionally low (1 μL) injection volumes of surrounding media into either 100% hydrogel, liquid media or any other medium. It automatically measures morphology before and after the 채취 and transfer to prove that the organoids retain their morphology and structure due to the very gentle transfer method.
Real-time tracking of changes in 오가노이드 eccentricity (object roundness) and darkness (object brightness) with the Incucyte® Live-Cell Analysis System enables rapid, unbiased assessment of optimal culture passage periods.
The iQue®3 spheroid immune cell killing (ICK) workflow measures subset analysis and cytokines quantification to examine the activation, killing, 탈진, and memory profiles of T cells during co-culture with single spheroids. The workflows utilize the iQue®3 High-Throughput 선별 Cytometer with associated suite of T cell characterization reagent kits, and validated spheroid washing and dissociation protocols to provide an end-to-end solution.
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