Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Poster

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly scaling each process step.

Traditionally Single Radial Immunodiffusion (SRID) assays have been used as the ‘gold standard’ but require very skilled operators to obtain reproducible results and is relatively low throughput. ELISAs have also been used to determine titre but have lower precision and dynamic range. Bio-Layer Interferometry (BLI) technology, used on Octet® systems, combines the highthroughput characteristics of a 96-well plate based ELISA assay in conjunction with improvements in accuracy and repeatability derived from a simpler direct measurement of mass transfer on binding.

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