Unique Mixed Mode Selectivities for Chromatography Solutions
New biotherapeutics, including nanobodies, bispecific mAbs, and fusion proteins, present challenges to traditional downstream purification platforms. These novel biopharmaceutical targets require specific selectivities to achieve product purity. Additionally, higher mAb titers and newly engineered mAb-like fusion proteins drive the need for increased productivity and selectivity in purification processes. Mixed mode chromatography combines multiple interaction types (e.g., ionic, hydrophobic, affinity) on a single ligand to overcome these challenges. These features:
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Mixed mode chromatography combines multiple interaction types, including anion exchange (AEX), cation exchange (CEX), hydrophobic interaction (HIC), and affinity chromatography, on a single ligand to improve separation efficiency and reduce process steps. Explore our mixed mode solutions to find the right fit for your purification challenges.
Unique Selectivity with Mixed Mode Resins
Achieve superior separation of complex proteins with mixed mode resins.
Hydrophobic
Pseudo-affinity for mAbs
Capture of mAbs, sdAbs, Fabs, enzymes, and recombinant proteins
Purification of proteins such as IgG, Fab, alpha-chymotrypsin, glutathione S-transferase (GST), and fibrinogen
Removal of host cell proteins, DNA, aggregates, and variants
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The unique selectivity of mixed mode monolithic columns ensures the proper purification of the most challenging large biomolecules.
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Achieve highly selective purifications with our affinity chromatography solutions.
Optimize your separation process with ion exchange resins, membranes, and monoliths.
Browse a range of hydrophobic interaction chromatography media to suit your 어플리케이션.
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Discover high DBCs, low-salt binding, and selective separation for optimal sdAb capturing.
싸토리우스 mixed mode resins offer enhanced selectivity by combining ion exchange, hydrophobic interaction, and affinity chromatography. These resins support a broad range of pH and ionic strength conditions without requiring pre- or post-diafiltration steps, enabling more streamlined, intensified processes.
This study demonstrates the efficacy and versatility of MEP HyperCel as a capture platform for a wide variety of single-domain antibodies (sdAbs). sdAbs, also known as VHH fragments or nanobodies, are camelid-derived heavy-chain antibodies with unique structural and functional properties. Unlike full-length mAbs, which can be easily purified using Protein A affinity chromatography, sdAbs lack a universal binding domain, making their purification more complex and highly dependent on tailored solutions.
MEP HyperCel offered:
CMM HyperCel also proves to be a valuable option, particularly for acidic sdAbs requiring near-neutral pH elution, offering flexibility in process optimization.
Further refinement of binding conditions at low salt concentrations, along with optimized contact times and DBC, may allow even greater process efficiency using the MEP and CMM HyperCel capture platform. This work was performed in collaboration with VALIDOGEN, a leading contract R&D organization (CRO) specializing in recombinant protein expression using its proprietary UNLOCK PICHIA® expression system.
“Mixed mode” or “multimodal” refers to a resin’s ability to interact with molecules through multiple binding mechanisms simultaneously. The majority of 싸토리우스 mixed mode resins combine ion exchange (IEX) and hydrophobic interaction chromatography (HIC) to enhance separation performance. The mechanism separates biomolecules first by their isoelectric point and then by their relative hydrophobicity, providing an additional dimension of selectivity.
Mixed mode resins act as a “safety belt” in downstream processing when:
Value of Mixed Mode Resins:
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