Speed Up Your Entire Workflow with iQue® 5 High-Throughput 선별 (HTS) Cytometry

Screen a 96-Well Plate in 5 Minutes 

Traditional flow cytometers can analyze a 제한 number of samples but fall short for rapid screens of antibodies or chimeric antigen receptor (CAR) T cells.

The iQue® HTS Cytometer addresses this critical gap as a data generation powerhouse for high-throughput 선별 applications. Its unique advantages lie in its speed and user-friendliness, from setup to acquisition and analysis, dramatically boosting productivity. Your results will be ready by the time you finish a quick coffee break.

Featuring a patented sampling method, the iQue® enables the fastest sample acquisition in the industry. It supports 96- or 384-well plates and facilitates continuous plate loading through integration with popular robotics systems. Coupled with integrated analysis and novel visualization tools, this platform delivers quality results faster, so you can develop therapeutics sooner. 

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Choose the Right iQue® HTS Cytometer for Your Needs

Get the Data You Need Faster

  • Market-leading speed completes processes 96-well plates in as few as 5 minutes or 384-well plates in 20 minutes
  • Validated consumables and buffers ensure consistent performance
  • Plug and play kits speed up assay design and 밸리데이션
  • Patented sampling with replaceable probe / tubing reduces downtime from clogs 

Design Your Assay Your Way     

  • Broad detection range covers cells, beads, yeast and more (down to 0.2 µm)
  • Up to 27 channel capacity supports wide range of applications
  • Flexible throughput by compatibility with 96- and 384-well plates
  • Integration with robotics systems allows extended operation 

A True User-Friendly Experience

  • Intuitive iQue Forecyt® Software simplifies operation for users of all levels
  • Multi-user environment features walkaway automation, comprehensive analysis, and advanced visualization tools
  • iQue® Qmax trolley provides convenient sheath and waste fluid management during long runtimes

System Overview: iQue®  HTS Cytometer

Explore the Wide Range of Supported Applications

Antibody Discovery and Development

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Adoptive 세포 치료제

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Small Molecule 선별

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  • A simple, scalable, multi-user environment featuring walkaway automation, comprehensive analysis and visualization tools that are not available in traditional flow cytometers
  • Walk-up-and-use operation that eliminates the need for pre-analytical user setup and power users
  • Flexible plate formats suitable for a broad range of cell and bead-based analyses 

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Rapid, Walkaway Processing of  96 or 384 Well Plates

  • Patented, rapid 마이크로볼륨 샘플링 processes 96 well plates in as few as 5 minutes or 384 well plates in 20 minutes
  • Samples are delivered to detectors in an air-gap delimited stream, so an entire plate of data can be processed at one time
  • Generous cartridge station allows for unattended multiple plate processing

Simultaneous Cell Phenotyping and Secreted Protein Detection


Real-time Data Acquisition and Analysis and Dynamic Visualization of Results

  • Rapid assay optimization – adjust a 게이트 to see updated plate-wide analysis in real time
  • Easy, interactive assay development and decision environment that is reproducible across plates   
  • Plate level annotation, analysis, and visualization integrated within the 표준 population selection tools

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HTS by Cytometry Features

Biologically Relevant, Reproducible, and Cost Effective HTS Cytometry

Speed

Relieve capacity bottlenecks throughout your entire workflow with faster plate sampling, integrated analysis, and novel data streamlining tools. 

   

Simplicity

Enjoy an easy to use, multi-user environment with walkaway automation, including automated QC bead vortexing on-board and 48-hour continuous run times.  

   

Scalability

Take advantage of the open platform design for integration with leading robotic systems for automatic plate handling and operation.

   

Expert Help

Rely on our experts to overcome hardware, software, or technical challenges and get your experiments back on track as fast as possible.

   

High-Throughput 선별 (HTS) by Cytometry Software, Reagents, and Consumables

High-Throughput 선별 (HTS) by Cytometry Software

iQue Forecyt® Software —Rapid Data Visualization and Interpretation

The integrated iQue Forecyt® software addresses the analysis bottlenecks associated with multi-parametric data generation by enabling rapid data visualization and interpretation.

Reduce data analysis times from days to minutes by having all of your data analyzed as soon as the plate is done sampling.

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유세포분석 Reagents & Consumables

Optimized for the iQue® HTS Platform

iQue® Kits, most of which have been developed to work with 노워시 protocols, are packaged to suit a wide range of throughput needs. 

These kits, in conjunction with an iQue® HTS Cytometer and iQue Forecyt® Software, provide an integrated 선별 solution enabling high content, high throughput 선별 of cells and beads for deeper insight into complex biologies.

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iQue® Specifications & Optical Configurations

The iQue® is Available In Multiple Optical Configurations and Flexible Plate Formats

The iQue® HTS by Cytometry Platform is available in 4 configurations to address a wide range of 선별 and experimental applications. The entry level 2 laser iQue® 3 BR is equipped with a blue and a red laser ideal for applications which do not require many parameters, but, can take advantage of the speed the iQue® HTS Platform is famous for.

3 laser configurations increase the flexibility and accommodate larger multiplexing needs depending on the specific fluorphores. The iQue® 3 VBR includes Violet, Blue, and Red lasers, while, the iQue® 3 VYB replaces the red laser with a yellow laser. 

The iQue® 5 further expands iQue® capabilities, with its 4 (Violet–Yellow-Blue–Red) laser and 27 channel configurations. 

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 iQue® 3 Blue-Red (BR)iQue® 3 Violet-Blue-Red (VBR)iQue® 3 Violet-Yellow-Blue (VYB)iQue® 5  Violet – Yellow -Blue – Red
Catalog No.91375, 91381 (HD)9137791379BA-97135
Lasers488nm, 640nm 405nm, 488nm, 640nm405nm, 561nm, 488nm405 nm, 488 nm, 561 nm, 637 nm
Detection Channels   8151527
Plate Types   96- and 384-well, 384- and
1536-well (Blue-Red HD)
96- and 384-well96- and 384-well96-and 384- well
Min Sampling Time< 5 minutes/96 wells, < 20 minutes/384 wells
Min Sample Aspiration1 µL1 µL1 µL1 µL
Detection RateUp to 35,000 events/second
Dynamic Range> 7 Decades7.2 Decades
Clogging Detection Bubble sensor to visually ID Clogs

 

Optional ProductsCat. No.
Forecyt® 엔터프라이즈 에디션 with 5 Floating Client Licenses91131
로보틱 통합 API License90208
QMax WorkstationRequest Info

 

High-Throughput 선별 (HTS) by Cytometry Applications

High-Throughput 선별 (HTS) by Cytometry Applications to Support Your Most Important Research 

To efficiently characterize disease states and to develop a clear picture of how single cells respond to a stimulus, whether it’s a small molecule, antibody, 사이토카인, or other entity, quantitative measurement of multiple biological readouts is required. 

iQue® HTS by Cytometry Applications enable the assessment of multiple cell types and a range of cellular parameters in a single well, allowing for a streamlined high-throughput workflow, improved data coherence and a highly informed data set.


Request a Quote or Demo  Explore All High-Throughput 선별 (HTS) by Cytometry Applications  

Antibody Characterization

Increase data throughput and quality by multiplexing antibody binding, function, and 역가 across the process.

사이토카인 Profiling

Assess multiple cell parameters faster, with fewer cells and less reagents.

Cell Monitoring and Workflows

Streamlined combined workflows and protocols that are simple and easy to follow providing greater biological insights

Immune Cell Function

Understand the Complexities of the Immune System with the iQue® HTS Platform

Cell Health & Proliferation

Analyze cell health, viability and proliferation on a cell-by-cell basis with the iQue® HTS Platform

Related Products and Resources

Product Resources

Brochure

iQue® 5 High-Throughput 선별 (HTS) by Cytometry

PDF | 3.3 MB

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High-Throughput 선별 (HTS) by Cytometry Applications

Applications for live-cell, multiplexed analysis of cell phenotype and function in a single well, to resolve complex cellular questions.

ForeCyt Software

iQue Forecyt® Software

Designed for speed, iQue Forecyt® is a comprehensive all-in-one package, including plate level annotation, analytics, and visualization.

flow cytometry reagents

High-Throughput 선별 (HTS) Reagents

Most reagents have been developed to work with 노워시 protocols, and are validated and packaged to suit a wide range of throughput needs.

Explore More Resources

eBook

Second Edition High-Throughput 선별 (HTS) by Cytometry Handbook

A Guide to iQue® High-Throughput 선별 by Cytometry, Assays and Workflows.

Webinar

The Importance of Immune Profiling in  CAR-T Therapies

Cytometry in the introduction of CAR constructs into alternative immune cells, and studies in gene modified cells which target solid tumors.

적용분야 노트

T Cell Activation and Function Characterization in Cancer Immunotherap...

Characterization of T Cell Activation and Function in Cancer Immunotherapy using High-Throughput Cytometry

Additional Resources

iQue® High-Throughput Cytometry Publications

With over 1,200 references worldwide, the iQue®️ HTS Platform helps researchers explore new areas of study and address questions fundamental to life science research. 

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iQue® Cytometry Panel Builder

Use the dynamic, interactive panel design tool, for streamlining the creation of complex panels, to profile populations on different iQue® configurations.. 

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