플라스미드 Engineering and Sequencing Services
Plasmids are DNA vectors that remain critical material for the development of a wide range of advanced biotherapies: 유전자 치료제 (AAV, LV), 유전자 변형 세포치료제 (CAR-T), mRNA-based therapies and more. Plasmids control the expression of genes of interest to be transferred in a biological system for research, bio-processing or therapeutic 어플리케이션. By optimizing 플라스미드 design, researchers and developers can enhance the safety, efficacy, and specificity of biotherapies, ultimately improving treatment outcomes. Accessible through our intuitive online platform, 플라스미드 engineering is powered by our unique and innovative DNA assembly technology (e-Zyvec®). Thanks to its flexibility and accuracy, our technology simplifies the generation of any type of 플라스미드, from the easiest to the most sophisticated. Tailor-designed plasmids are also easily transferable to our 플라스미드 manufacturing processes to obtain higher quantities of higher grade (up to GMP) materials.
싸토리우스 offers a modular, user-friendly platform for designing custom plasmids, with expert support and technology that ensures precise assembly—even for complex or novel constructs. For simpler needs, we provide pre-designed plasmids through a fast, cost-effective service that includes sequencing and discovery-grade pDNA preparation. To guarantee accuracy, all engineered plasmids undergo thorough 밸리데이션 using in-house Oxford Nanopore-based NGS platform, which is also available for customer DNA analysis.
We offer an innovative modular approach to 플라스미드 design, applicable to any type of 플라스미드. With our user-friendly 플라스미드 platform, you can freely design your plasmids, focusing on your sequences of interest. Our experts will then review your design and advise you, when required, about relevant ways to improve your molecules.The e-Zyvec® technology ensures that each 플라스미드 assembled accurately contains all your sequences, including repeated sequences, high or low GC contents, long sequences, or multiple transgenes. If the 플라스미드 you need does not exist yet, we can make it for you.
Design My Plasmids
For the most basic needs, we also offer a range of simple plasmids, available on our 플라스미드-services platform . Pre-designed plasmids are provided at competitive costs and shortened turn-over times.
What’s included in this offer?
Explore Easy 플라스미드
Small sequence modifications, such as a single point mutation, can impair the biological functionality of a 플라스미드, thus jeopardizing downstream prcesses. To mitigate this risk as much as possible, we have developed our own Next Generation Sequencing analytical platform based on ONT, so all our engineered plasmids can be delivered with a comprehensive sequencing report..This capacity is also made accessible to our customer for NGS 밸리데이션 of their own DNA molecules. Our bioinformatics combine long-read mapping and assembly applicable to either circular or linear DNA molecule.
Personalized studies using either ONT or Illumina technologies are also available through service contract. Please inquire.
Create Your Account Graphical representation of the sequencing data across the entire reference sequence of the sample, highlighting potential mutations and sequencing depth.
Designed as a one-stop-shop for 플라스미드 services, our free of access online design platform is simply accessible from your MySartorius account. With our intutive 플라스미드 configurator, you will be able to start new projects for 플라스미드 engineering, sequencing, and more. This is the most direct way to get in contact with our project management experts and request quotes for our 플라스미드 services.
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We deliver “discovery grade*” plasmids as circular, double stranded DNA molecules, in a screw-cap plastic tube, re-suspended in 1X AE buffer (miniprep.) or 1X TE buffer (midi, maxi, giga prep.), at room temperature. Please note that we DO NOT provide bacterial stock.
Discovery grade plasmids can be ordered at multiple formats, please inquire for more information.
For larger scale or higher quality grade 플라스미드 production please inquire 플라스미드 Manufacturing Service
*Discovery grade means that while plasmids are produced in a sterile environment according to good laboratory practice, quality control are 제한 to basic quality and identity test. Discovery grade plasmids should be restricted to Research 어플리케이션.
Upon receipt, briefly spin down the tubes containing your engineered plasmids (bench centrifuge) and store them at:
We strongly recommend to 분액 the Polyplus discovery grade plasmids for long storage and to avoid freeze-thaw cycles.
Duration of storage are given here as indication. You should always control the integrity of long stored plasmids by running gel electrophoresis (denaturation) or sequencing (sequence integrity).
We do offer a pDNA 증폭 service for either engineered plasmids we generated, or your own 플라스미드 you may wish to send us.
증폭 can be performed at small scale (up to several mg) and discovery grade, or at large scale (up to several gram) at R&D, High Quality or GMP grade with our 플라스미드 Manufacturing Service.
You can send your samples following the guidelines below:
Samples should be sent to:
Polyplus-핵산전달감염Production Team80 rue du Dr Yersin59120 LoosFRANCE
When known, please enter the sequences of reference of your samples on our platform.
After sequencing your sample, we will provide you the full-length sequence of your 플라스미드 (.fasta file) as well as a sequencing report (.pdf file) containing:
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