Setting The 표준 in Lentiviral Solutions for 유전자 치료제 and 유전자 변형 세포치료제, Together
Scientists often use lentiviral (LV) vectors to deliver genes to therapeutic cells (gene-modified cell therapies) and offer potentially life-saving treatment against cancers or delivered directly to patients to treat rare genetic disorders. Because developers can use LV as a raw material or as the final therapy product, it’s critical to build robust processes that achieve consistently high viral yields with high quality.
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Explore lentiviral vector process solutions to simplify progress in every step of your workflow.
: Media handling is complex, and adventitious agents risk contaminating your media or buffer.
: Simplify and accelerate your media preparation and sterile connections.
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: Meet regulatory requirements with reliable impurity testing for your single-use systems.
filters: Ensure sterile filtration using sterilizing-grade heterogeneous double-layer filters available in 0.8 and 0.45 μm pre-filter and a 0.2 μm 최종 필터 membrane.
: Mitigate viral contamination using a viral retention filter designed for cell culture media.
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: Suboptimal 핵산전달감염 conditions can lead to low 역가, reduced 바이러스 벡터 quality, and scaling challenges.
: Design your own plasmids and optimize your 바이러스 벡터 production with our equipment, raw materials, and services.
: Process optimization during development and scale-up is manual and time-consuming.
: Enable fast and accurate process optimization using optimized analytics — including design of experiments (DOE) and quantification of total virus particles - and ensure robust process control with 다변량 데이터 분석 (MVDA) software.
: Accelerate progress by quickly tapping into the power of DOE.
: Improve process understanding, model complex systems, and gain the deep process understanding needed to drive growth.
Platform: Quantify total virus particles quickly and easily using a flow virometer combined with Virotag® DY ENV or Virotag® VSVG reagent.
: Monitor the effects of feed strategy and media components on cell culture performance.
: Lentivirus is a large, enveloped virus that can get trapped in filters and damaged.
: Achieve high lentivirus recovery with a fast and scalable filter train.
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prefilters: Enhance particle reduction with high-performance and low-adsorptive polypropylene fleece prefilters. We recommend using a two-stage filter train starting with a retention rate of 20 μm followed by 0.65 μm.
0.45 μm: Enhance total throughput and flow rate performance by combining 0.8 μm and 0.45 μm filter pore sizes with 미생물오염(도) and particle-retentive elements.
: Lentivirus is fragile with a short half-life.
: Concentrate lentiviral vector gently and efficiently using Hydrosart® high-performance membrane cassettes.
: Use of Hydrosart® cellulose based membrane for low LV adsorption and good recovery using 100 or 300KDa MWCO.
: Screen conditions and optimize candidate selection with a high-throughput TFF solution for parallel 선별.
, , and : Optimize ultrafiltration and diafiltration applications downstream from process development to manufacturing clinical trials.
: Lentivirus has a short half-life, and infective particles can have low recovery rates.
: Support fast flow rate using membrane and monolith technologies.
: Freezing and thawing biological products improperly can undermine your product’s quality and effectiveness.
: Confidently store and transport sensitive drug products using controlled freeze-thaw and validated shipping solutions.
: Filtration can involve inconsistent filling accuracy, low product recovery, and contamination risk.
: Maintain product integrity and sterility with cutting-edge filtration technologies.
To help find the right materials and consumables for your needs, request a sample from our portfolio.
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Achieve high recovery, scalability, and fast processing time using Sartobind® Convec D for lentivirus capture.
Increase LV 역가 and particle quality with optimized 핵산전달감염 reagents and plasmids.
Manage your entire cold chain with confidence and reach high LV functional titers.
Stabilize your lentiviral 유전자 치료제 with versatile recombinant human albumin.
Preserve sterility during connection and disconnection throughout your biomanufacturing process.
Increase lentivirus recovery using closed, pre-sterilized, and low-adsorption membranes with MWCO of 100 or 300 kDa.
Protect your product during storage and shipping for sterile filtration, media preparation, sampling, and fluid transfer steps.
Preserve sterility and protect your process from virus contamination with reliable filters for media and buffer filtration.
Generate high lentivirus yield and 역가 with scalable and widely compatible media.
Achieve a sterile product by using a high-efficiency, low-adsorption, and robust filter to maximize yield.
Optimize your lentivirus process for larger volumes with a fully scalable, single-use bioreactor.
Browse LV 유전자 치료제 products by searching or filtering by product type.
Lentiviruses are a family of viruses insert DNA into the host cells' genome upon transduction. They can permanently integrate genetic material into dividing and nondividing cells. Lentiviral vectors are produced from a culture of packaging cells, which scientists then transfect with plasmids. The vector-producing cells are then expanded in culture and purified.
Lentiviruses are transducing, dividing, and nondividing cells, and enable long-term expression by integrating into the cell genome. They have a large payload of 9.7 kb. Lentivirus is unique in its ability to infenct nondividing cells, and has high transduction toward targets like T-cells.
BSL2. Scientists can't perform viral clearance on lentiviruses using a filter, so single-use and closed systems are prefered. Scientists need to control raw materials and filter them to remove virus. Removing impurities like DNA and HCP is difficult because lentiviruses are large and sensitive, making downstream process challenging.
This webinar showcases the optimization of a scalable LV production process using a high-throughput, automated microbioreactor&nbs...
This poster demonstrates a process development approach for using CIM® monolithic columns in the downstream purification of lentiviruses.
Learn early PD strategies that enhance clinical-stage production of AAV and LV.
Read about how this project design allowed for rapid deployment of processes and scale-up to a 50 L Biostat STR®.
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